1985年硕士毕业于华中农业大学微生物学专业，1994年获得美国马里兰大学植物分子生物学专业博士学位，2002年以来任新加坡基因组学研究院副院长、高级学科带头人，全球3D基因组学创始人。长期从事基因组学研究，具有创建世界知名研究院的经历, 见证、参与并推动了基因组学研究的发展历程，基因组学研究领域国际知名战略科学家。多次应邀在冷泉港（CSHL）、Keystone等国际知名学术大会上作特邀报告。在2012年人类全基因组计划完成十周年之际，受邀在Science杂志刊载点评，回顾了基因组学的研究进展并展望了其未来。同年入选Cell子刊Cell Report编委组。

在国际上率先发明了基因组学研究新策略，建立了当今世界最先进的基因组学研究方法体系----全基因组双末端标记测序系统（Paired-End-Tag，简称PET），并在此基础上衍生开发了ChIP-PET、RNA-PET和DNA-PET等多种技术平台。以上系统化的平台不仅可以解释非传统的融合转录物及结构异常，而且使得研究者能够从基因组结构变化到转录组调控层面深入展示人类功能基因组信息。 2009年，申请人首次在国际上建立了揭示基因组内远程调控信息及染色质三维拓扑结构的先进技术平台-ChIA-PET (Chromatin Interaction Analysis using Paired End Tag sequencing) 技术平台。以上这些独特的技术平，为生命科学家在未来5-10年描绘出细胞内的人类基因组序列的空间三维图谱及解密疾病发生的本质奠定了强有力的技术基础。为揭秘长链非编码RNA（lncRNA)与DNA的三维调控网络在人类生物学过程中功能机制，团队再次建立了RICH-PET技术，为破译lncRNA在肿瘤发生发展过程中的调控功能提供了尖端平台。申请人在新加坡国立基因组研究院每年固定研究经费超过1000万美元，并先后获得美国国家卫生研究院（NIH）、美国国防部（DOD）等研究项目经费逾600万美元。在美国杰克逊实验室期间不仅获得NIH资助的RICH-PET项目及美国国家人类基因组研究所（NHGRI）资助ChIA-PET workshop等多项基金，而且为杰克逊医学基因组学实验室首次获得NIH授权的四维核组学（4D Nucleome）研究中心。多年来共发表了103篇高水平的学术论文，其中包括Nature、Science、Cell、Nature Genetics、Nature Methods、PNAS、 Genome Research 等等。仅自2005年以来，第一/通讯作者影响因子400分以上。

目前阮一骏教授课题组研究焦点集中在整合现有独特的多种技术平台， 从基因组结构、染色质与染色质之间的远程调控及染色质与lncRNA的三维转录调控网络等多层面，全面揭秘及剖析不同肿瘤（其中包括白血病、乳腺癌及肝癌）发生、发展及转移恶化过程中的异常调控机制，并确定肿瘤治疗过程中潜在的分子靶标，从而为肿瘤患者精准医疗方案的实施提供坚实的理论依据及索引。

教育经历

1978-09-01至1982-02-01    华中农业大学，微生物学，大学本科

1982-09-01至1985-06-30    华中农业大学，微生物学，硕士

1990-08-01至1994-12-01    马里兰大学 University of Maryland，分子生物学，博士

工作经历

作为项目负责人主持的国家级及省部级课题：

U54 DK107967 Ruan (PI) 09/30/2015-07/31/2020

Nucleome Positioning System for Spatiotemporal Genome Organization and Regulation

The goal of this program is to develop robust and efficient 3D genome-mapping technologies that can be broadly applied to address biological questions related to the topological organization and regulatory functions of genomes.

Role: PI

R25 HG007631 Ruan (PI)        09/01/2015-06/30/2018

Workshop of Chromatin Interaction Analysis Using Pair-End Tag Sequencing 11/15-14/2017

The goal of this 5-day workshop was to train young scientists (graduate students, post-doctoral trainees, new investigators) in the use of ChIA-PET as a tool for asking questions about chromatin interactions, 3D genome organization and genome regulation in human health and disease, with a balance of didactic and laboratory-based instruction.

R01 CA186714-01 Ruan (PI)   04/01/14-03/31/17

Characterization of RNA-Chromatin Interactome by RNA-DNA Ligation and Sequencing

The study will identify novel non-coding RNAs (ncRNAs) and the interactions between ncRNAs and their target DNAs.  

Completed Research Support

The Jackson Laboratory TJL-IRF-FY13-YR-CD-JHC    Ruan (PI) 09/01/13-08/31/15 Epigenetic and Transcriptional Responses to Environmental Stresses

The goals were to study for comprehensive epigenomic comparison of genome-wide transcriptional regulatory responses to arsenic exposure and to elucidate the role of HSF1 in epigenetic regulation.  

The Jackson Laboratory TJL-IRF-FY13-YR-KP Paigen, Ruan (PI) 09/01/13-08/31/15 The Role of Non-coding RNAs in Meiosis

The goal was to apply RICh-PET to study the roles of long non-coding RNAs in meiosis.  

代表性著作及论文

1. Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, … , Sung WK, Liu ET, Wei CL, Cheung E, Ruan Y* (2009). An oestrogen-receptor-α-bound human chromatin interactome. Nature 462: 58-64. (IF:38.138)

2. Zhang Y, Wong CH, Birnbaum RY,…, Ruan Y, Wei CL. (2013). Chromatin connectivity maps reveal dynamic promoter-enhancer long-range associations. Nature. 504(7479):306-10. doi: 10.1038/nature12716. (IF: 38.138)

3. Yijun Ruan* (2011) Genome-sequencing anniversary. Presenting the human genome: now in 3D! Science 2011 Feb 25; 331(6020): 1025-6. doi: 10.1126/science.1203602. (IF: 34.661)

4. Xu Q, ..., Ruan Y* (2012) The draft genome of sweet orange (Citrus sinensis). Nature Genetics. 45(1):59-66. (IF: 31.616)

5. Yuin-Han Loh, ..., Yijun Ruan*, Bing Lim, Huck-Hui Ng*, (2006) The Oct4 and Nanog transcription network that regulates pluripotency in mouse embryonic stem cells. Nature Genetics 38: 431-440. (IF: 31.616)

6. Edwin Cheung and Yijun Ruan* (2011) Determination of transcription factor binding. Nature Genetics 43: 11-12 (January 2011) (IF: 31.616)

7. Lusy Handoko, ..., Yijun Ruan*, Chia-Lin Wei* (2011) Functional Genome Architecture Revealed by CTCF-Associated Chromatin Interactome in Pluripotent Cells. Nature Genetics 43: 630–638 (June 19, 2011) . (IF: 31.616)

8. Li G,..., Ruan Y*. Extensive promoter-centered chromatin interactions provide a topological basis for transcription regulation. Cell. 2012;148(1-2):84-98. (IF: 28.71)

9. Kieffer-Kwon KR, ..., Ruan Y, Casellas R.Interactome maps of mouse gene regulatory domains reveal basic principles of transcriptional regulation. Cell 2013;155(7):1507-20. (IF: 28.71)

10. Tang Z, ..., Ruan Y* (2015). CTCF-Mediated Human 3D Genome A, rchitecture Reveals C, hromatin Topology for Transcription. Cell, 163(7): 1611–1627. (IF: 28.71)

11. Wei CL, ..., Ruan Y*. A global map of p53 transcription-factor binding sites in the human genome. Cell. 2006; 124(1):207-19. (IF: 28.71)

12. Ng P, ..., Ruan Y*. Gene identification signature (GIS) analysis for transcriptome characterization and genome annotation. Nature Methods 2005; 2(2):105-11. (IF: 25.328)

13. Patrick Ng, Chia Lin Wei, Yijun Ruan* (2006) Paired-End diTagging for Transcriptome and Genome Analysis. Current Protocols in Molecular Biology UNIT 21.12.

14. Ruan Y*,..., Wei CL. (2007) Fusion transcripts and transcribed retrotransposed loci discovered through comprehensive transcriptome analysis using Paired-End diTags (PETs). Genome Research 17(6): 828-838. (IF:11:351)

15. Wei Chia Lin, ..., Ruan Yijun* (2004) 5"Long serial analysis of gene expression (LongSAGE) and 3" LongSAGE for transcriptome characterization and genome annotation. Proc Natl Acad Sci U S A. 101:11701-11706. (IF: 9.423)

发明专利：

1. Yijun Ruan, Method for gene identification signature (GIS) analysis (4371967), 2009, Singapore, (Granted)

2. Yijun Ruan, Method to generate or determine nucleic acid tags corresponding to the terminal ends of DNA molecules using sequence analysis of gene expression (terminal SAGE) (155774), 2009, Singapore, (Granted)

3. Yijun Ruan, Method to generate or determine nucleic acid tags corresponding to the terminal ends of DNA molecules using sequence analysis of gene expression 5 & 3 LongSAGE (GB2409457), 2006, Singapore, (Granted)